auditory cued fear learning  (Med Associates Inc)

Journal: Genes

Article Title: Effects of Paraquat, Dextran Sulfate Sodium, and Irradiation on Behavioral and Cognitive Performance and the Gut Microbiome in A53T and A53T-L444P Mice.

doi: 10.3390/genes15030282

Figure Lengend Snippet: Figure 11. (A) In the open field, activity levels were higher in A53T than A54T-L444P mice (F(1,81) = 4.091, * p = 0.046). (B) In the rotorod test, there was a genotype × treatment interaction (F(1,81) = 7.773, p = 0.007). DSS impaired rotorod performance in A53T-L444P mice (F(1,45) = 9.228, *** p = 0.004), but not in A53T mice. (C) DSS reduced spontaneous alternation in A53T-L444P mice (F(1,45) = 7.191, * p = 0.01). (D) A53T-L44P mice showed reduced activity levels in the Y-maze (F(1,81) = 5.336, * p = 0.023). (E) During the baseline period in the fear conditioning test, activity levels were lower in A53T-L44P than A53T mice (F(1,81) = 7.565, ** p = 0.007). (F) In males, A53T-L44P mice showed lower activity levels than A53T mice during the baseline period (F(1,41) = 6.727, * p = 0.013). (G) In females, there was a treatment × genotype interaction (F(1,40) = 4.168, p = 0.048). DSS increased activity level in A53T-L44P females (F(1,22) = 5.119, * p = 0.034). (H) For the freezing levels during the ISI, there was a genotype × treatment × sex interaction (F(1,81) = 4.661, p = 0.034). (I) A53T-L44P mice had lower activity levels than A53T mice during the ISI (F(1,81) = 4.564, * p = 0.036). (J) When activity levels during the tones were analyzed, there was a genotype × treatment × sex interaction (F(1,81) = 8.199, p = 0.005). (K) A53T-L44P mice had lower activity levels than A53T mice during the contextual fear memory test (F(1,85) = 4.677, * p = 0.033). Circles: A53T; squares: A53T-L444P. Open symbols: H2O; filled symbols: DSS.

Article Snippet: Contextual and cued fear conditioning were assessed (Med Associates mouse fear conditioning system (PMED-VFC-NIR-M, Med Associates, St. Albans, Vermont and Med Associates VideoFreeze automated scoring system).

Techniques: Activity Assay

Journal: Science (New York, N.Y.)

Article Title: Female-specific synaptic dysfunction and cognitive impairment in a mouse model of PCDH19 disorder

doi: 10.1126/science.aaz3893

Figure Lengend Snippet: (A to G) Fear conditioning test. (A) Schematic of the contextual and cued fear conditioning test. Context A is different from context B. CS, white noise; US, 0.3 mA foot shock. (B to G) Freezing responses of female (B to D) and male (E to G) mice. (B and E) Freezing responses on day 1. CS was presented for 30 s, 3 times, (gray shadow) and at the end of each CS, a US was given (red). (C and F) Freezing responses on day 2 (contextual test). (D and G) Freezing responses on day 3 (cued test). CS was presented at 3–6 min (gray shadow). Freezing responses during the conditioning, contextual and cued tests were similar between Pcdh19 mutant and control mice. n = 14 mice for Pcdh19WT♀, 10 for Pcdh19HET♀, 12 for Pcdh19WT♂, and 12 for Pcdh19HEMI♂.

Article Snippet: Contextual and cued fear conditioning tests, pre-exposure dependent fear facilitation (Pattern completion) task, contextual discrimination fear learning (Pattern separation) task, and distinct contextual fear conditioning tests were conducted in a conditioning chamber (Ugo Basile) equipped with a digital video camera and a sound-attenuating chest.

Techniques: Mutagenesis, Control